FIG. 6.

FMB were compared with commercial Biosilon microcarriers for their performance as hMSC carriers and allowing cell survival at room temperature. The proportion of cells attached to the two types of beads was assayed by MTS on the first day, and the rate of proliferation of the cells in the incubator in the CO₂ incubator at 37°C was assayed at different time points. The cells attached to the two types of beads were sealed at room temperature according to the protocol just mentioned. Initial cell attachment on Biosilon and FMB and the proliferation rate are shown in (A). Comparison of the survival of hMSCs on FMB and Biosilon after sealing of cells on beads for 6 days at room temperature is shown in (B). The range of data for the hMSC on FMB is taken from the detailed data presented in Figure 3A. PI fluorescence for nuclei staining of cells on FMB or Biosilon with dim light before (day 1) (C) and after sealing in room temperature (day 7) (D), demonstrate the cell density on these beads *p<0.05, **p<0.01. Color images available online at www.liebertonline.com/tec