FIG. 11.

(A) Optical DIC image sequence (8 days) showing initial seeding of HPCF population, (B) first cell layer formation (cells elongated in direction of arrows), and then (C) second layer formation of cells on a disorganized construct. The last frame (D) depicts a generally organized sheet-motion of one layer of HPCFs sliding over a second layer of cells. Movies of the population dynamics and the cell sheet motion are available in Supplementary Data. One can see numerous phenomena, including the rate of proliferation of cells during the entire observation period.