Four different thermal performance test runs on the bioreactor under conditions of varying flow rate. The target center chamber temperature was 37°C. Solid lines represent the average temperature from the two control thermocouples on either side of the chamber (labeled: Boundary). The dashed line represents the temperature of the center of the bioreactor. All curves represent experimental runs in triplicate. (A) No flow, Center: 36.6°C±0.13°C, Control points: 37.7°C±0.11°C. (B) 4 μL/min, Center: 36.6°C±0.09°C, Control points: 37.7°C±0.07°C. (C) 40 μL/min, Center: 36.5°C±0.08°C, Control points: 37.6°C±0.08°C. (D) 400 μL/min Center: 36.5°C±0.06°C, Control points: 37.4°C±0.04°C.