Quantification of accumulated sulfated glycosaminoglcyan (sGAG) content a) within cell-laden PEG hydrogels and b) which was released into the culture medium for free-swelling (FS) and dynamically loaded conditions. In c), the % change in sGAG release due to loading is presented (i.e., % change is defined as the difference in sGAG released between the loaded constructs and the free swelling constructs relative to the free swelling constructs). PEG hydrogels contained no additives (◻), hyaluronan (∎), Link-N (∎), or hyaluronan and Link-N (∎). Data are represented as mean ± standard deviation. ND indicates none detected, * indicates difference from free-swelling controls p<0.05, † indicates significant difference from PEG-only gels containing no additives p<0.05.