Figure 6.

The density of myofibers is increased in diaphragm muscle from mdx mice treated with 1D11 or losartan. Cryosections from diaphragm muscle were stained with Alexa Fluor 488-labeled wheat germ agglutinin to visualize the sarcolemma and with DAPI to visualize the nuclei. Fibers were quantitated by their size distribution in microns (horizontal axis) using image analysis software. Data are reported as means. Statistical analyses were by analysis of variance followed by Dunnett's multiple comparison test relative to vehicle control. *P < 0.01; n = 8 per group.