Skip to main content
. Author manuscript; available in PMC: 2011 Jun 27.
Published in final edited form as: Virology. 2006 Oct 30;359(2):425–435. doi: 10.1016/j.virol.2006.09.044

Fig. 1.

Fig. 1

Creation of the ΔvIL-6 recombinant virus. (A) Strategy for generation of the ΔvIL-6 recombinant virus. The structure of the first 30 kb of the KSHV genome is shown at the top. Viral genes are indicated as open boxes. Most of the vIL-6 gene was deleted as described in the Materials and methods. Genomic structure of the resulting reconstituted KSHV-GFP1 and ΔvIL-6 is shown. (B) Southern blot analysis of virion DNA isolated from the supernatants of induced BCBL-1 cells and Vero cells infected with KSHV-GFP1 or ΔvIL-6. The probes used are shown in panel A. Probe P1 corresponds to the vIL-6 region deleted (BC-1 positions 17089 to 17563). Probe P2 corresponds to the remaining genomic region of vIL-6 and full length of ORF2. (C) Northern blot analysis of uninduced and induced KSHV-GFP1 Vero cells and ΔvIL-6 Vero cells. BCBL-1 cells and Vero cells infected with KSHV-GFP1 or ΔvIL-6 recombinant viruses were induced with Adeno-50 virus and sodium butyrate. Forty-eight hours post induction, mRNA was extracted and analyzed. The Northern blot was probed with radiolabeled sequences containing viral IL-6, PAN RNA and GAPDH, respectively. U: uninduced. I: induced with Adeno-50 virus and sodium butyrate.