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. Author manuscript; available in PMC: 2011 Jun 27.
Published in final edited form as: Virology. 2006 Oct 30;359(2):425–435. doi: 10.1016/j.virol.2006.09.044

Fig. 4.

Fig. 4

Evaluation of Lytic reactivation of KSHV-GFP1 and ΔvIL-6 recombinant KSHV in BJAB cells. BJAB cells containing KSHV-GFP1 or ΔvIL-6 were induced with Adeno-50 recombinant virus and 3 mM sodium butyrate. Cells were fixed and stained for flow cytometry 24 h after induction. The top panel lists the percentage of live cells expressing ORF 59. The bottom panel shows an example of the primary flow cytometry data. The x axis is set for GFP detection, while the y axis is set for ORF59 antibody conjugated to phycoerythrin. The gate shown was based on negative and positive controls, and applied to all samples.