Table 3.
Laboratory analysis methods and their sensitivity and linearity
| Substance | Measurement method | Linearity | Sensitivity | Dialysate (mean ± SD) [min–max] | Blood (mean ± SD) [min–max] |
|---|---|---|---|---|---|
| Urea (mmol/L) | UV cinetic | 0.8–50 | 0.38 | 4.6 ± 1.4 [1.3–9.3] | 12 ± 8 [1.6–31] |
| Creatinine (μmol/L) | Compensated Jaffe | 5–2000 | 2.4 | 85 ± 2 [23–173] | 406 ± 230 [73–939] |
| β2 microglobulin (mg/L) | Immunoturbidimetry | 0.5–16 | 0.06 | 6.5 ± 7.1 [0.06–37] | 13.5 ± 5.6 [3.2–23] |
| Total proteins (dialysate) (g/L) | Photometric colour | 0.01–2.00 | 0.007 | 0.10 ± 0.06 [0.01–0.44] | |
| Total proteins (dialysate) (mg/L) | Bradford | 0–500 | 0.002 | 98 ± 60 [0.002–272] | |
| Total proteins (serum) (g/L) | Photometric colour | 30–120 | 0.77 | 67 ± 7 [52–84] | |
| Albumin ‘micro’ assays (mg/L) | Immunoturbidimetry | 5–300 | 0.46 | 12 ± 14 [0.1–113] | |
| α1-Antitrypsin (g/L) | Immunoturbidimetry | 0.3–5.0 | 0.01 | 1.5 ± 0.3 [0.84–2.44] | |
| Leptin (μg/L)a | ELISA | 0.0156–1 | 0.0078 | 25 ± 47 [0.6–386] | |
| RBP (mg/L)a | ELISA | 1.56–100 | 0.224 | 13 ± 7 [1.4–44] | 178 ± 96 [55–667] |
a
Leptin and RBP were diluted before measurement in order to be in the linearity range.