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. 2011 Jun;178(6):2654–2664. doi: 10.1016/j.ajpath.2011.02.043

Figure 6.

Figure 6

Fibrogenic gene expression in cultured ocular fibroblasts. A: Adding exogenous TGFβ1 up-regulated TRPV1 mRNA expression in WT ocular fibroblasts. B: Loss of TRPV1 reduced the mRNA expression level of TGFβ1 in ocular fibroblasts. C: Expression of mRNAs of IL-6 was markedly up-regulated by adding exogenous TGFβ1, but such up-regulation was abolished by the loss of TRPV1 gene in the fibroblasts. Expression of MCP-1 (D) and vascular endothelial growth factor (VEGF) (E) also was suppressed in ocular fibroblasts by lacking the TRPV1 gene, and the expression pattern was not affected by exogenous TGFβ1. F: There was no difference in the expression level of SP mRNA between cultured WT and KO ocular fibroblasts, and the expression pattern was not affected by exogenous TGFβ1. G: Expression of mRNAs of collagen Iα1, the major fibrogenic marker, was up-regulated by adding exogenous TGFβ1, but such up-regulation was abolished by the loss of TRPV1 gene in the fibroblasts. H: Expression of mRNAs of α-SMA, a myofibroblast marker, was markedly up-regulated by adding exogenous TGFβ1, but such up-regulation was completely abolished by the loss of TRPV1 gene in the fibroblasts. I: Fibronectin was suppressed in ocular fibroblasts by lacking TRPV1. Adding exogenous TGFβ1 up-regulated fibronectin in WT ocular fibroblasts, but such up-regulation was abolished by the loss of TRPV1 gene in the fibroblasts. Data represent mean ± SEM from five specimens in each condition. *P < 0.05. Scale bar = mean ± SEM. GAPDH, glyceraldehyde-3-phosphate dehydrogenase.