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. Author manuscript; available in PMC: 2011 Jun 27.
Published in final edited form as: Mol Microbiol. 2010 Feb;75(4):924–941. doi: 10.1111/j.1365-2958.2009.07027.x

Fig. 2. PcrV requires an intact secretion signal to control effector export.

Fig. 2

(A) a pcrV deletion mutant was complemented with either wild-type pcrV, a pcrV deletion mutant lacking codons 3–21, or a fusion in which codons 1–21 of exoS were fused to codons 22–294 of pcrV. The ability of the indicated ORF to complement the pcrV null mutant phenotype was monitored using a lacZ reporter inserted in the exoS locus (A) or by RECC assay (B). The ability of the indicated open reading frames to complement the defect in cytotoxicity of a pcrV null mutant was assayed by monitoring rounding of A549 cells after 5 hours of infection (MOI 10)(C).