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. Author manuscript; available in PMC: 2011 Jun 27.
Published in final edited form as: Mol Microbiol. 2010 Feb;75(4):924–941. doi: 10.1111/j.1365-2958.2009.07027.x

Fig. 4. Co-immunoprecipitation of PcrG and PcrV.

Fig. 4

A functional, VSV-G-tagged version of PcrG (PcrG(i3VG)) was used to determine the localization of PcrG in the cell. Bacteria were fractionated using the lysozyme/EDTA method. Fractions are indicated above each lane (wc = whole cell, S = supernatant, C = cytoplasm, P = periplasm, M = membrane). Fractionation was controlled using markers for secreted proteins (PcrV), membrane proteins (OprH), periplasmic proteins (beta-lactamase) and the cytoplasm (RpoA).