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. Author manuscript; available in PMC: 2011 Jun 27.
Published in final edited form as: Mol Microbiol. 2010 Feb;75(4):924–941. doi: 10.1111/j.1365-2958.2009.07027.x

Fig. 7. Phenotypic characterization of pcrG point mutants.

Fig. 7

The effect of mutating the chromosomal copy of pcrG was assayed by β-galactosidase assay, using a lacZ reporter inserted in the exoS locus (A), or by RECC assay (B). Proteins detected in the RECC assay are indicated to the left of each blot, the fraction to the right. (C) Stability of PcrG was assayed by modifying the chromosomal copy of pcrG to express an internally VSV-G tagged version of PcrG (PcrG(i3VG)). The western blot was performed on cell pellets of bacteria grown under T3SS inducing (low calcium) conditions.