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. Author manuscript; available in PMC: 2011 Jun 27.
Published in final edited form as: Mol Microbiol. 2010 Feb;75(4):924–941. doi: 10.1111/j.1365-2958.2009.07027.x

Fig. 8. PcrV-binding and regulatory functions of PcrG can be separated.

Fig. 8

Full length PcrG or truncated versions (aa 2–40 or 41–95) were fused to the C-terminus of MBP and expressed in a mutant of P. aeruginosa lacking pcrG. The ability to control effector export was monitored by β-galactosidase assay using a lacZ reporter inserted into the chromosomal exoS locus (A). The ability of the MBP-fusions to bind to PcrV was determined by pull-down from cytoplasmic extracts using an amylose-resin, followed by Western blot analysis (B). Secretion of PcrV was analyzed by RECC assay (C). The genotype and plasmid is noted above each set of lanes. Supernatant and cell pellet fractions, as well as the protein being detected are listed to the side of the relevant panel.