| BL21(DE3) Codon+ - RP |
E. coli B F– ompT hsdS(rB– mB–) dcm+ Tetr gal λ (DE3) endA Hte [argU proL Camr] |
Stratagene |
| KDZif1∆Z |
two-hybrid analysis strain lacking rpoZ (encoding ω) and harboring a test promoter-lacZ fusion to detect Zif-dependent two-hybrid interactions |
(Vallet-Gely et al., 2005) |
| RP1831 |
PAO1F, wild type P. aeruginosa PAO1 |
(Bleves et al., 2005) |
| RP1868 |
PAO1F ∆exoS::GFP-lacZ
|
this study |
| RP3082 |
PAO1F ∆pcrG2 ∆exoS::GFP-lacZ
|
this study |
| RP2645 |
PAO1F ∆pcrV2 ∆exoS::GFP-lacZ
|
this study |
| RP3335 |
PAO1F ∆pcrGV2 ∆exoS::GFP-lacZ
|
this study |
| RP3666 |
PAO1F pcrG(A16R) ∆exoS::GFP-lacZ
|
this study |
| RP3466 |
PAO1F pcrV(L262D) ∆exoS::GFP-lacZ
|
this study |
| RP3468 |
PAO1F pcrV(F279R) ∆exoS::GFP-lacZ
|
this study |
| RP3898 |
PAO1F pcrG(i3VG) ∆exoS::GFP-lacZ
|
this study |
| RP3900 |
PAO1F pcrG(i3VG, A16R) ∆exoS::GFP-lacZ
|
this study |
| RP4348 |
PAO1F pcrG(i3VG) pcrV(L262D) ∆exoS::GFP-lacZ
|
this study |
| RP4349 |
PAO1F pcrG(i3VG) pcrV(F279R) ∆exoS::GFP-lacZ
|
this study |
| RP3929 |
PAO1F ∆exsE ∆fleQ pcrG(i3VG) |
this study |
| RP3770 |
PAO1F ∆exsE ∆fleQ ∆pcrV2
|
this study |
| plasmid |
relevant features |
reference |
|
| pPSV37 |
colE1 origin, gentR, PA origin, oriT, lacUV5 promoter, lacIq, stops in every reading frame preceding the MCS and T7 terminator following the MCS (relative to the lacUV5 promoter) |
this study |
| pEXG2 |
Allelic exchange vector, colE1 origin, oriT, gentamycin resistance, sacB
|
(Rietsch et al., 2005) |
| pDuet1 |
Co-expression vector with T7 origin, bla resistance |
Novagen |
| pACTR-AP-Zif |
pACYC origin, tetR, plasmid allowing for the creation of N-terminal fusions to Zif, the zinc-finger DNA binding domain of the murine Zif268 protein, under control of a lac promoter |
(Vallet-Gely et al., 2005) |
| pBRω |
colE1 origin, bla, plasmid allowing for the creation of N-terminal fusions to the ω subunit of RNA polymerase under control of a lac promoter |
(Vallet-Gely et al., 2005) |
| pZifVG |
pACTR-AP-Zif modified to include a linker encoding the VSV-G tag between the fusion partner and the Zif moiety (see Experimental Procedures) |
this study |
| pMal |
pPSV37 encoding a signal-sequenceless malE gene (codons 27–396) lacking a stop codon followed by a polylinker to create MBP fusions |
this study |
| pP37-pcrG
|
pcrG under control of the lacUV5 promoter in pPSV37 |
this study |
| pP35-pcrV
|
pcrV under control of the lacUV5 promoter in pPSV35 |
this study |
| pP37-pcrV
|
pcrV under control of the lacUV5 promoter in pPSV37 |
this study |
| pP37-pcrGV
|
pcrGV under control of the lacUV5 promoter in pPSV37 |
this study |
| pP35-pcrV(∆3–21) |
pcrV lacking codons 3–21 under control of the lacUV5 promoter |
this study |
| pP35-exoS(1–21)-pcrV(22–294) |
codons 1–21 of exoS fused to codons 22–294 of pcrV, expressed under the control of the lacUV5 promoter |
this study |
| pEXG2-∆exoS::GFP-lacZ |
allelic exchange vector which deletes exoS and inserts translationally coupled versions of GFP and lacZ in its place |
(Rietsch et al., 2004) |
| pEXG2-∆pcrG2
|
allelic exchange vector designed to delete codons 6–88 of pcrG
|
this study |
| pEXG2-pcrG(ATG1-) |
allelic exchange vector to change the annotated ATG of pcrG to ATC |
this sudy |
| pEXG2-pcrG(ATG2-) |
allelic exchange vector to change the second ATG (codon 4 of the annotated sequence) to GGG |
this study |
| pEXG2-pcrG(i3VG) |
allelic exchange vector designed to replace the chromosomal copy of pcrG with a version of the open reading frame in which codons 55–57 have been replaced by 3 tandem repeats of the VSV-G tag |
this study |
| pEXG2-pcrG(i3VG, A16R) |
allelic exchange vector designed to replace the chromosomal copy of pcrG(A16R) with a version of the open reading frame in which codons 55–57 have been replaced by 3 tandem repeats of the VSV-G tag |
this study |
| pEXG2-∆pcrGV2
|
allelic exchange vector designed to delete pcrG and pcrV starting at codon 6 of pcrG and ending at codon 180 of pcrV
|
this study |
| pEXG2-pcrV(L262D) |
allelic exchange vector designed to introduce the L262->D mutation into pcrV
|
this study |
| pEXG2-pcrV(F279R) |
allelic exchange vector designed to introduce the F279->R mutation into pcrV
|
this study |
| pEXG2-pcrG(A16R) |
allelic exchange vector designed to introduce the A16->R mutation into pcrG
|
this study |
| pACZif-mvaU
|
two-hybrid plasmid encoding a MvaU-Zif fusion protein |
(Vallet-Gely et al., 2005) |
| pBRω-mvaT
|
two-hybrid plasmid encoding a MvaT-ω fusion protein |
(Vallet-Gely et al., 2005) |
| pAcZif-pcrG
|
two-hybrid plasmid encoding a PcrG-Zif fusion protein |
this study |
| pBRω-pcrV
|
two-hybrid plasmid encoding a PcrV-ω fusion protein |
this study |
| pBRω-pcrV(L262D) |
two-hybrid plasmid encoding a PcrV(L262D)-ω fusion protein |
this study |
| pBRω-pcrV(F279R) |
two-hybrid plasmid encoding a PcrV(F279R)-ω fusion protein |
this study |
| pZifVG-pcrG
|
two-hybrid plasmid encoding a PcrG-VSV-G tag-Zif fusion protein |
this study |
| pZifVG-pcrG(A16R) |
two-hybrid plasmid encoding a PcrG(A16R)-VSV-G tag-Zif fusion protein |
this study |
| pDuet-pcrG
|
T7 promoter expression vector for the expression of an amino-terminally His-tagged version of PcrG |
this study |
| pDuet-pcrV
|
T7 promoter expression vector for the expression of an untagged version of PcrV |
this study |
| pDuet-pcrGV
|
T7 promoter expression vector for the concomitant expression of an amino-terminally His-tagged version of PcrG and an untagged version of PcrV |
this study |
| pDuet-pcrG(A16R) pcrV
|
T7 promoter expression vector for the concomitant expression of an amino-terminally His-tagged version of PcrG(A16R) and an untagged version of PcrV |
this study |
| pDuet-pcrG pcrV(L262D) |
T7 promoter expression vector for the concomitant expression of an amino-terminally His-tagged version of PcrG and an untagged version of PcrV(L262D) |
this study |
| pDuet-pcrG pcrV(F279R) |
T7 promoter expression vector for the concomitant expression of an amino-terminally His-tagged version of PcrG and an untagged version of PcrV(F279R) |
this study |
| pMal-pcrG
|
plasmid encoding an MBP-PcrG fusion protein under control of a lacUV5 promoter |
this study |
| pMal-pcrG(2–40) |
plasmid encoding an MBP-PcrG(aa 2–40) fusion protein under control of a lacUV5 promoter |
this study |
| pMal-pcrG(41–95) |
plasmid encoding an MBP-PcrG(aa 41–95) fusion protein under control of a lacUV5 promoter |
this study |
