Fig. 6.

Decreased expression and activity of β1 integrin in 4.1R^−/− keratinocytes. (A,B) Expression of integrins in keratinocytes. The surface expression of β1, α5, α6 and β4 integrins was measured by flow cytometry. The representative profiles and quantitative analysis (the mean fluorescence intensity ± s.e.m from three independent experiments) are shown in A and B, respectively. For simplicity, autofluorescence control from only wild-type cells is shown. (C,D) Activity of β1 integrin in keratinocytes. The active form of β1 integrin in the absence or presence of Mn²⁺ was stained by anti-mouse CD29 and measured by flow cytometry. The representative profiles are shown in C and quantitative data from three experiments is shown in D. (E) Western blot analysis of β1 integrin in keratinocytes. 20 μg protein of total lysates was probed with indicated antibodies. GAPDH immunoblot is shown as a loading control. (F) Quantitative analysis from three independent experiments.