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. 2011 May 17;6:1027–1034. doi: 10.2147/IJN.S18023

Table 1.

Summary of primer sequences for bcl-2, bax, caspase 3, survivin, and GAPDH

Gene Primer for RT-PCR Band size (bp) Annealing temperature (°C)
bcl-2 Forward 5′-TGTGGCCTTCTTTGAGTTCG-3′ 281 56
Reverse 5′-TCACTTGTGGCCCAGATAGG-3′
bax Forward 5′-TTTGCTTCAGGGTTTCATCC-3′ 246 52
Reverse 5′-CAGTTGAAGTTGCCGTCAGA-3′
caspase 3 Forward 5′-TTGATGCGTGATGTTTCTA-3′ 223 51
Reverse 5′-CAATGCCACAGTCCAGTTC-3′
survivin Forward 5′-CTCAAGGACCACCGCATCTC-3′ 392 55
Reverse 5′-CCTCAATCCATGGCAGCCAG-3′
GAPDH Forward 5′-GCCACATCGCTCAGACAC-3′ 614 53
Reverse 5′-CATCACGCCACAGTTTCC-3′

Abbreviation: RT-PCR, reverse transcriptase polymerase chain reaction.