Table 1.
Gene | Primer for RT-PCR | Band size (bp) | Annealing temperature (°C) |
---|---|---|---|
bcl-2 | Forward 5′-TGTGGCCTTCTTTGAGTTCG-3′ | 281 | 56 |
Reverse 5′-TCACTTGTGGCCCAGATAGG-3′ | |||
bax | Forward 5′-TTTGCTTCAGGGTTTCATCC-3′ | 246 | 52 |
Reverse 5′-CAGTTGAAGTTGCCGTCAGA-3′ | |||
caspase 3 | Forward 5′-TTGATGCGTGATGTTTCTA-3′ | 223 | 51 |
Reverse 5′-CAATGCCACAGTCCAGTTC-3′ | |||
survivin | Forward 5′-CTCAAGGACCACCGCATCTC-3′ | 392 | 55 |
Reverse 5′-CCTCAATCCATGGCAGCCAG-3′ | |||
GAPDH | Forward 5′-GCCACATCGCTCAGACAC-3′ | 614 | 53 |
Reverse 5′-CATCACGCCACAGTTTCC-3′ |
Abbreviation: RT-PCR, reverse transcriptase polymerase chain reaction.