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. 2011 May 20;12:23. doi: 10.1186/1471-2091-12-23

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Copyright ©2011 Arbuckle et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Nuclear localization of GFP-tagged non-core RAG1 domains. HeLa cells were transfected with GFP fused to the N-terminus of the indicated RAG1 polypeptides. Following 24-48 hrs cell growth, cells were fixed and stained with DAPI. Left panels: fluorescence microscopy images of HeLa cells expressing (A) GFP-full length RAG1, (B) GFP-core RAG1, (C) GFP-CND (residues 87-217), (D) GFP-bZDD (residues 218-380), and (E) GFP-(CND+bZDD) (residues 87-380). Right panels: The corresponding nuclei of cells in the left panels stained with DAPI.