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. 2011 May 20;12:23. doi: 10.1186/1471-2091-12-23

Table 1.

Zinc:Protein Ratio for CND and bZDD

RAG1 Domain Conditions Zinc:Protein Ratio ICP-MSa,b Zinc:Protein Ratio FAASa,b
CNDc Zinc Free Buffer 2.08 ± 0.68 2.00 ± 0.44
CNDc DTPA Buffer 0.24 ± 0.14 0.37 ± 0.29
bZDDd Zinc Free Buffer 4.23 ± 0.65 4.12 ± 0.78

a The zinc content of each construct was determined using ICP-MS and FAAS.

b Data presented represent the average of two or more independent experiments.

c Zinc binding results for mbp-CND and tagless CND were comparable. The results presented for CND represent the average from both data sets.

d Zinc binding for bZDD was evaluated for the mbp-tagged construct only.