Figure 1.

Effect of CpG-DNA encapsulated in different liposome complexes on IL-8 promoter activation. Human RPMI 8226 cells (left) and mouse RAW 264.7 cells (right) were transiently transfected with pIL-8-Luc for 24 h and stimulated with indicated liposomes or CpG-DNA (PO-ODN) encapsulated in the indicated liposome complexes for 12 h. Cultures were harvested and assayed for luciferase assay. The luciferase activity of the cells was measured in relative light units, and this value was normalized to Renilla activity. The results are represented as fold activation and compared to the PBS-treated control. Each bar represents Mean ± SD values of three experiments. *P < 0.05, **P < 0.01 (vs PBS control).