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. 2011 May 18;12:29. doi: 10.1186/1471-2172-12-29

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Copyright ©2011 Kim et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Induction of TLR9-dependent epitope-specific IgG production by a complex of peptide and Lipoplex(O). BALB/c mice (WT) and BALB/c TLR9 knockout mice (TLR9-/-) (n = 3/group) were immunized with hTM4SF5R2-3 peptide (50 μg/mouse) and Lipoplex(O) complex (Lipoplex(O) + TM4SF5R2-3) (a), IFA with 50 μg of HEL (b), or IFA with hTM4SF5R2-3 peptide (b). The sera were collected, and amounts of the peptide-specific total IgG were assayed with an ELISA kit. The results are expressed as a Mean ± SD of three mice. This experiment was performed 2 times with similar results. *P < 0.05 (vs control mice).