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Comparative analysis of amyloid fibril formation by wild-type and Trp95Leu variant. A) Thioflavin assay of fibrils formed after incubation for 72 hours. B) Upper panels: light microscopy image at 10 × magnification of Congo red stained wild-type and Trp95Leu variant β2-m after incubation for 72 hours (same material as in Figure 7A). Lower panels: polarized light microscopy image of Congo red stained β2-m amyloid fibrils. The apple green birefringence is characteristic of amyloid.
