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. 2011 Jun 27;6(6):e21258. doi: 10.1371/journal.pone.0021258

Figure 5. Localization of PDF and ITP neuropeptides in the optic lobe of Drosophila melanogaster.

Figure 5

Flies were examined at four ZTs: ZT1, ZT4, ZT13 and ZT16 (ZT0 – the beginning of the day and ZT12 – the beginning of the night) but images shown in Fig. 5 were obtained from the brain of individuals collected for experiments at different ZTs. ZT for each image is given in brackets. A1–3: PDF- immunoreactive (magenta) and labeled with cry-GAL4-driven GFP (green) neurons. The 5th s-LNv is PDF-negative and PDF is also not present in CRY-positive projection invading the lamina (arrow) (ZT16). B1–3 (ZT1), C1–3: ITP-immunoreactive (magenta) and labeled with cry-GAL4-driven GFP (green) neurons. ITP- immunoreactivity co-localizes with CRY-positive processes in the lamina. The magenta staining in the retina is non-specific while in the medulla, ITP- immunoreactivity is both specific and unspecific (ZT13). RE – retina, LA – lamina, ME – medulla. Scale bars: 20 µm.