Table 2. Results from the sequencing and quantification of samples.
| Sample | Copy #/µL | S.D. | Amplication # | Direct Sequence | Majority Rules | CCP | Clones |
| 1 | 14285 A | 14285 A | 14285 A | 13:14285A; 1:14285N | |||
| 809005 | 644 | 9.784 | 2 | 14285 A | 14285 A | 14285 A | 10:14285A; 1:14285A, 14293T; 1:14285A, 14322A |
| 3 | 14285 A | NA | NA | Not cloned | |||
| 1 | Reference | Reference | Reference | 14:Reference; 1:14285N, 14289N; 1:14300N | |||
| 809007 | 1737 | 332.96 | 2 | Reference | Reference | Reference | 14:Reference; 1:14210A, 14227A, 14231A, 14247A, 14316T; 1:14241T, 14244T, 14245T, 14246T, 14284T |
| 3 | Reference | NA | NA | Not cloned | |||
| 1 | Reference | Reference | Reference | 15:Reference; 1:14223G | |||
| 809016 | 91 | 29.132 | 2 | Reference | Reference | Reference | 15:Reference; 1:14334A |
| 3 | Reference | NA | NA | Not cloned | |||
| 1 | Reference | Reference | Reference | 10:Reference; 1:14210A, 14281A,14339N; 1:14227A, 14265A, 14280A, 14304A: 1:14281N | |||
| 809023 | 35 | 3.868 | 2 | 14227N, 14231N, 14265N, 14269N, 14281N | None | None: Did not meet 95% confidence | 5:14227A, 14231A, 14265A, 14269A, 14281A, 14304A, 14334A; 3:14227A, 14231A; 2:14210A, 14211A, 14265A, 14281A, 14299A, 14232A,14233A; 1:14227A, 14231A,14265A, 14269A, 14281A, 14304A; 1:14210A, 14211A, 14334A; 1:14265A, 14269A,14334A; 1:14280A, 14281A, 14299A, 14304A, 14334A; 1:14265A, 14269A, 14281A, 14304A, 14334A |
| 3 | Reference | NA | NA | Not cloned | |||
| 1 | 14285A | 14285A | 14285A | 11: 14285A; 2:14227A, 14285A; 1:14250N, 14285A; 1:142545T, 14285A; 1:14285A, 14334A | |||
| 809032 | 115 | 42.496 | 2 | 14285A | 14285A | 14285A | 14:14285A; 1:14227A, 14285A; 1:Reference |
| 3 | 14285 A | NA | NA | Not cloned |
Samples were sequenced from nps 14207–14345, relative to a complete mtDNA genome, NC_008415 [42], and polymorphisms listed (not in bold) are relative to that reference sequence. Consensus sequences have been submitted to Genbank and assigned accession numbers HQ95713-HQ95717. For the clone category, results should be read as follows: the first number refers to the number of clones that have the damage/error, the second number provides the position in the sequence followed by the letter of the base pair that is now seen (e.g. 13:14285A reads as 13 clones with an adenine present at site 14285). Quantification results represent the average copy number over duplicate qPCR reactions and the standard deviation is reported. Note that qPCR results are indicative of the relative DNA level, but should not be taken as exact quantification.