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. 2011 Jun 27;6(6):e21341. doi: 10.1371/journal.pone.0021341

Figure 4. Porcine 4Ig-B7-H3 inhibited T cell functions.

Figure 4

A, Freshly isolated porcine PBMCs were seeded to 96-well plate precoated with 1 µg/ml of anti-CD3 mAb plus either 10 µg/ml of 4Ig-B7-H3-Fc or Fc in 50 µl over night at 4°C. After culturing for 72 hours, T cell proliferation in the presence of Fc (left plot) or 4Ig-B7-H3-Fc (right plot) was analyzed. The plots were from one representative experiment of three independent samples. B, Statistic analysis of mean fluorescence intensity (MFI) on T cells (mean ± s.e.m). C and D, T cells purified from porcine PBMCs were seeded to the 96-well flat-bottomed plate precoated with 1 µg/ml of anti-CD3 mAb plus either 10 µg/ml 4Ig-B7-H3-Fc or Fc in 50 µl over night at 4°C. After culturing for 72 hours, supernatants were harvested to quantify IFN-γ (C) and IL-2 (D) concentration using ELISA kit. All data were from a representative of three independent experiments. * means P<0.05.