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. 2011 Jun 27;6(6):e21452. doi: 10.1371/journal.pone.0021452

Figure 4. Graphical representation of the SLC4A3 protein.

Figure 4

A) Both isoforms are made up of a transmembrane domain flanked on both sides by a cytoplasmic domain (represented by C*). The number of amino acids that make up each domain are indicated. Several amino acids at the N-terminus are unique to either the full-length (280 residues) or cardiac (73 residues) isoform, but the remainder of the protein is identical in both isoforms. B) The locations of the two nonsynonymous SNPs in the confirmed canine SLC4A3 transcript. C) The cardiac isoform alone is affected by the A44C SNP mutation (p.Q15P). Both isoforms are affected by the 2601_2602insC mutation. 867 amino acids at the N-terminus of the full-length protein and 660 of the cardiac isoform are normal. However the insertion causes a shift in the reading frame of 104 amino acids, leading to a premature termination codon. This results in a truncated protein product, lacking 266 residues of the C-terminus of both isoforms, if expressed. Therefore a large part of the transmembrane region and the entire c-terminal cytoplasmic region is absent in the mutant proteins.