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(a) Real time amplification curve of β-actin transcripts from total universal RNA at various concentrations. The curves from left to right correspond to 0, 0.02, 0.2, 1, and 10 pg/nl of RNA. (b) Evolution in Ct values vs the RNA concentration obtained in microdroplets (round symbols) and, in a conventional manner, in the Lightcycler 480.
