Figure 1. Hypoxic culture conditions upregulate 3BP2 gene expression.

Subconfluent MSC were serum-starved and cultured under normoxic (5% CO₂ and 95% air; white box) or hypoxic (1% O₂, 5% CO₂ and 94% N₂; black box) conditions for 18 hours. Total RNA was extracted and quantitative reverse transcription-polymerase chain reaction was performed in order to assess 3BP2, HIF-1α, VEGF and MT1-MMP gene expression levels. Values are means of two independent experiments, each performed in triplicates. Bars, ±SD.