Figure 4. Effects of insulin stimulation and glucose concentration on lactate secretion of human astrocytes.

Prior to experiment, NHAs were either starved in medium containing 1 g/l glucose or kept in medium with 4.5 g/l. Medium was than replaced by new medium with the same glucose concentrations without (white bars) or with 50 nM insulin (grey bars). After 8 hours, lactate concentration in the supernatant was measured. Bars represent means of five independent experiments + SEM. There were no significant differences between groups (ANOVA, p = 0.4).