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After starvation for at least 24 hours, cells were treated with the indicated concentrations of insulin for 3 days. Thereafter, cell proliferation was assessed by WST-1 assay. Bars represent means of six independent experiments + SEM. There were significant differences between the groups (ANOVA, p = 0.0007). * indicates significant difference from 0 nM (Tukey Kramer post hoc test p<0.05).
