Figure 2. Different modes of self-renewal by individual RGLs in the adult dentate gyrus.
(A) Time course of activation of labeled RGLs in the adult dentate gyrus after induction with the 1X dose of tamoxifen. Shown is the quantification of clones consisting of a single RGL, indicating quiescence. Values present mean ± SEM (n = 3–8 animals).
(B–F) Sample confocal images of GFP+ cells in the process of, or right after RGL cell division (Pc ≥ 99.8%). Shown are samples of neurogenic asymmetric cell division (B, D), astrogenic asymmetric cell division (C, E) and symmetric cell division (F). The potential lineage relationship is indicated in the insert. R: RGL; N: neuronal lineage; A: astroglia. Note that after cell division, the RGL and newborn astroglia returned to quiescence (MCM2−; D, E), whereas the IPC re-entered the cell cycle (MCM2+; D). Scale bars: 10 µm.
(G) A schematic diagram of three modes of self-renewal made by an RGL in the adult dentate gyrus.