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. Author manuscript; available in PMC: 2012 Jun 26.
Published in final edited form as: Biochem Biophys Res Commun. 2011 May 27;410(1):91–96. doi: 10.1016/j.bbrc.2011.05.112

Figure 4. Capn4 is essential for Galectin-3 secretion.

Figure 4

A) Western blot of galectin-3 from media of wildtype MEF (MEF-WT), Capn1 silenced MEF (Capn1 KD MEF), Capn2 silenced MEF (Capn2 KD MEF) and Capn4 −/− MEF cell (Capn4 −/− MEF). B) Calpastatin overexpressing MEF cells secrete Galectin-3 at levels similar to mock-nucleofected MEF cells (lanes labeled as MEF-WT and Calpastatin O/E - MEF). C) Capn4 −/− MEF cells rescued by exogenous expression of Calpain 4 secrete galectin-3 as compared to Capn4 −/− cells indicated as lane Capn4−/− MEF. Lanes indicated as Capn4−/− res 24 hr, Capn4−/− res 48 hr, Capn4−/− res 72 hr shows results from conditioned media collected from Calpain 4 rescued cells 24, 48 and 72 hours post nucleofection. The amount of secreted galectin-3 decreases as the proportion of Calpain 4 rescued cells is overgrown by Capn4−/− cells.