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. Author manuscript; available in PMC: 2012 Jul 1.
Published in final edited form as: Mol Microbiol. 2011 May 18;81(1):206–218. doi: 10.1111/j.1365-2958.2011.07686.x

Figure 6. GRASP is required for normal GXM secretion and capsule assembly.

Figure 6

A. India ink counterstaining of C. neoformans cells. Yeast strains are indicated on the top of each panel. B. Reactivity of C. neoformans cells with calcofluor white (blue fluorescence) and a monoclonal antibody raised against GXM (green fluorescence). Scale bars in A and B represent 20 and 10 µm, respectively. C. Determination of capsule size of the C. neoformans cells illustrated in A and B. GXM determination in culture supernatants (D) and infected brains (E) are shown, indicating that C. neoformans GRASP ortholog mutant shows a reduced content of extracellular GXM. Statistical analysis of the results shown in C, D and E indicates that values obtained for the GRASP ortholog mutant are significantly smaller than those found for WT and complemented systems (P<0.05 in all cases).