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. Author manuscript; available in PMC: 2012 Jul 1.
Published in final edited form as: Mol Microbiol. 2011 May 18;81(1):206–218. doi: 10.1111/j.1365-2958.2011.07686.x

Table 1.

List of primers used in this study.

Primer name Sequence (5’-3’) Purpose
CnGRASPF AAAATAGGGATAACAGGGTAATGAGATACCAGATGGACTGAA Disruption construct for GRASP1, 5’ flank
CnGRASP5R GGGGACAAGTTTGTACAAAAAAGCAGGCTATATATTCTGCCCAGCACATCT Disruption construct for GRASP1, 5’ flank
CnGRASP3F GGGGACCACTTTGTACAAGAAAGCTGGGTAATGCTAATGTGAAACGCAAT Disruption construct for GRASP1, 3’ flank
CnGRASP3R AAAAATTACCCTGTTATCCCTAGTAACGAGAAGTGCTGTCTC Disruption construct for GRASP1, 3’ flank
GRASPcompF ATAATGCAATGCGATGGCTGC Amplification of GRASP for complementation
GRASPcompR AATCCCCTCAAGAGCTCACGG Amplification of GRASP for complementation
RTGRASPF AGTTCTTTACCCTACTAGACAG Amplification of GRASP for RT-PCR
RTGRASPR TCTCCTCACATTGTCAGATTC Amplification of GRASP for RT-PCR
RTACTF CCTTCTACGTCTCTATCCAG Amplification of ACT1 for RT-PCR
RTACTR TTTCAAGCTGAGAAGACTGG Amplification of ACT1 for RT-PCR