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75Se incorporation into protein and tRNA. Cell extracts of wild-type Mm900 (lane 1), FSD10 (lane 2), and FSD10 containing the E. coli selD expression vector (lane 3), were prepared following growth on McCas medium in the presence of [75Se]selenite. Extracts were separated on a SDS/PAGE gel (4–20%), and the insertion of 75Se into macromolecules was followed by autoradiography. Migration position of 75Se-labeled RNA is indicated on the right.
