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. Author manuscript; available in PMC: 2012 Jul 1.
Published in final edited form as: Mol Microbiol. 2011 May 18;81(1):249–258. doi: 10.1111/j.1365-2958.2011.07690.x

Fig. 7.

Fig. 7

Transcription of genes encoding hydrogenase subunits in M. maripaludis Mm900, FHM1, and FSD10 strains, analyzed by RT-PCR. DNA-free total RNA from Mm900 wild-type (lanes 1, 4, 7, 10), or from deletion mutants carrying an insertion in the hrsM gene (lanes 2, 5, 8, 11) or the selD gene (lanes 3, 6, 9, 12) was subjected to RT-PCR. Primer pairs targeting fractions of the indicated genes encoding of the Se-free hydrogenases (frcA and vhcA), or on of the Se-containing hydrogenase (vhuD) were used. The primer pair directed against vhuD was also used in a control experiment where reverse-transcriptase has been omitted to exclude contamination with genomic DNA (lanes 10–12). The expected PCR product sizes were: frcA, 167 bp; vhcD, 165 bp; vhuD, 160 bp.