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. Author manuscript; available in PMC: 2012 Sep 1.
Published in final edited form as: Biochim Biophys Acta. 2011 May 5;1814(9):1134–1139. doi: 10.1016/j.bbapap.2011.04.015

Fig. 3.

Fig. 3

Phosphorylation of SMN in vitro by PKA. The purified GST-SMN was incubated with PKA or PKA pre-treated with PKA inhibitor (PKAI) in the presence of γ-32P-ATP at 30°C for 1 hour. Phosphorylation of GST-SMN was then detected by SDS-PAGE followed by film exposure. Some of the phosphorylated GST-SMN protein was subjected to phosphatase (PPase) treatment before gel electrophoresis.