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HPLC fractionation of ODC reaction with D-ornithine. (A) Control reaction without enzyme added. Diamines including putrescine and ornithine can react with multiple equivalents of the OPA reagent [18]. Although a large excess of OPA was used in an attempt simplify the chromatograms, the relatively high concentration of the diamines prevented complete derivatization. (B) Reaction with ODC and D-ornithine.
