Colon biopsies from patients with acute E. histolytica colitis were obtained from the sigmoid colon in an area of ulceration. Convalescent biopsies for comparison were obtained from the same patient 60 days later. RNA from colonic biopsy samples was isolated simultaneously from the same sample for microarray and RT-qPCR. (A) Comparison of microarray and (B) RT-qPCR REG 1A and REG 1B mRNA expression between colon biopsy samples from individuals with acute E. histolytica diarrhea (day 1) versus convalescence (day 60)