Figure 3.

Disk diffusion assays of detergent sensitivity for the P_fabHaF-fabHa(P₅)fabF control (HB13117), P_fabHaF-fabHa(P₅*)fabF (HB13118), sigW::spec (HB6208), fabHa::MLS (HB13127), fabHb::kan (HB13115), and P_xyl-fabF (HB13128) strains. Each bar represents the average zone of inhibition of at least three assays performed with three independent clones of each strain. The y axis shows the zone of inhibition (in millimeters), expressed as total diameter minus diameter of the filter paper disk (5.5 mm). Note that the scales of individual compounds vary for clarity. Error bars represent standard error. Student’s t tests were performed, and P₅*, sigW::spec, fabHb::kan, and P_xyl-fabF were found to be significantly different (P value ≤ 0.05) from the wild-type control for all four stresses. The P₅* and sigW::spec strains were also significantly different from each other under triton X-100, N-lauryl sarcosine, and amitriptyline treatment.

A. triton X-100

B. N-lauryl sarcosine

C. bile salts

D. amitriptyline