Table 1.
Stress | P5-lacZ | P5-lacZ ΔσM, V, X, Y, ylaC, Z | P5-lacZ ΔσW |
---|---|---|---|
Triton X-100 | +++ | +++ | - |
Vancomycin | ++ | ++ | - |
Polymyxin B | + | + | - |
Cephalosporin | + | + | - |
D-cycloserine | + | + | - |
The induction ability of the listed antibiotics was measured in the reporter strains HB13069 (P5-lacZ), HB13151 (P5-lacZ ΔsigM ΔsigY ΔsigZ ΔsigV ΔylaC ΔsigX), and HB13099 (P5-lacZ sigW::MLS) using disk diffusion assays (see Fig. S1 for examples). Blue halos were observed after overnight incubation: +++ (strong blue) > ++ (blue) > + (light blue) > - (white after 7 days, no induction). Each disk diffusion assay was performed at least three times with biological triplicates. Data shown is representative of all experiments.