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. Author manuscript; available in PMC: 2012 Jul 1.
Published in final edited form as: Mol Microbiol. 2011 Jun 9;81(1):69–79. doi: 10.1111/j.1365-2958.2011.07679.x

Table 1.

Summary of disk diffusion assays to monitor induction of P5-lacZ by cell envelope antibiotics

Stress P5-lacZ P5-lacZ ΔσM, V, X, Y, ylaC, Z P5-lacZ ΔσW
Triton X-100 +++ +++ -
Vancomycin ++ ++ -
Polymyxin B + + -
Cephalosporin + + -
D-cycloserine + + -
a

The induction ability of the listed antibiotics was measured in the reporter strains HB13069 (P5-lacZ), HB13151 (P5-lacZ ΔsigM ΔsigY ΔsigZ ΔsigV ΔylaC ΔsigX), and HB13099 (P5-lacZ sigW::MLS) using disk diffusion assays (see Fig. S1 for examples). Blue halos were observed after overnight incubation: +++ (strong blue) > ++ (blue) > + (light blue) > - (white after 7 days, no induction). Each disk diffusion assay was performed at least three times with biological triplicates. Data shown is representative of all experiments.