Table 2.
The effect of σW on membrane composition and fluidity
| Strain | W168 | PfabHaF- fabHa(P5)fabF Pxyl-sigW | PfabHaF- fabHa(P5*)fabF Pxyl-sigW | fabHa::MLS | fabHb::kan | Pxyl-fabF | fabHa::MLS Pxyl-fabF |
|---|---|---|---|---|---|---|---|
| % C15:0 iso a | 9.6 ± 0.6 | 6.5 ± 0.8 | 10.7 ± 1.4 | 1.8 ± 0.8 | 15.6 ± 0.3 | 6.5 ± 0.9 | 1.9 ± 0.2 |
| % C15:0 anteiso a | 40.0 ± 3.1 | 31.8 ± 3.2 | 42.2 ± 2.4 | 30.3 ± 1.9 | 39.0 ± 2.8 | 32.4 ± 3.1 | 30.0 ± 2.7 |
| % C16:0 iso a | 3.6 ± 0.4 | 2.3 ± 0.5 | 1.7 ± 0.5 | 5.2 ± 0.4 | 4.8 ± 0.8 | 3.5 ± 1.1 | 4.3 ± 0.4 |
| % C16:0 a | 8.3 ± 1.6 | 8.3 ± 0.5 | 7.5 ± 1.1 | 22.2 ± 1.8 | 6.8 ± 1.2 | 8.5 ± 1.6 | 16.8 ± 1.2 |
| % C17:0 iso a | 10.3 ± 2.0 | 9.0 ± 1.7 | 8.1 ± 0.7 | 4.8 ± 0.7 | 10.6 ± 2.2 | 11.9 ± 2.4 | 5.4 ± 1.0 |
| % C17:0 anteiso a | 26.0 ± 1.8 | 32.5 ± 0.8 | 24.8 ± 2.1 | 27.7 ± 0.7 | 20.3 ± 0.4 | 34.0 ± 2.0 | 35.4 ± 2.2 |
| % C18:0 a | 2.2 ± 0.6 | 9.6 ± 1.0 | 2.8 ± 0.8 | 7.8 ± 1.1 | 2.0 ± 0.7 | 3.3 ± 0.4 | 6.0 ± 0.9 |
| % straight FAs a | 10.5 ± 1.0 | 17.9 ± 1.2 | 10.3 ± 1.1 | 29.3 ± 0.7 | 8.8 ± 1.0 | 11.8 ± 1.8 | 22.0 ± 0.7 |
| % iso FAs a | 23.5 ± 2.9 | 17.8 ± 2.9 | 20.6 ± 1.6 | 11.4 ± 1.1 | 31.0 ± 2.0 | 21.9 ± 3.9 | 12.0 ± 1.3 |
| % anteiso FAs a | 66.0 ± 3.5 | 64.3 ± 3.9 | 67.0 ± 2.1 | 58.0 ± 1.5 | 59.3 ± 2.5 | 66.4 ± 4.0 | 65.7 ± 0.9 |
| 17:15 FA ratio a | 0.73 ± 0.07 | 1.08 ± 0.09 | 0.62 ± 0.08 | 1.03 ± 0.11 | 0.57 ± 0.08 | 1.18 ± 0.12 | 1.32 ± 0.22 |
| Fluorescence anisotropy analysisb | 0.185 ± 0.005 | 0.217 ± 0.003 | 0.202 ± 0.004 | ND | ND | ND | ND |
Data derived from FAME analysis of the following strains under xylose inducing conditions: W168, PfabHaF-fabHa(P5)fabF Pxyl-sigW (HB13121), PfabHaF-fabHa(P5*)fabF Pxyl-sigW (HB13122) fabHa::MLS (HB13127), fabHb::kan (HB13115), Pxyl-fabF (HB13128), and fabHa::MLS Pxyl-fabF (HB13132). Data is presented as the average of three trials (± standard error).
Data derived from fluorescence anisotropy analysis of the wild type (W168), PfabHaF-fabHa(P5)fabF amyE::Pxyl-sigW (HB13121), and PfabHaF-fabHa(P5*)fabF amyE::Pxyl-sigW (HB13122) strains under xylose inducing conditions. Data is presented as the average of three trials (± standard error). Student’s t-tests were performed, and all three values were found to be statistically different (P value ≤ 0.05) from each other.