General primers |
KOverifyUp F (1) |
CTTTAATGGGGCTTGGTATATGC |
KOverifyDwn R (2) |
GTTGATGCATCTGTTAAAATTG |
aad1-5′-R (3) |
TCCTTGAAGCTCGGGTATTA |
pKFSS1-3′F (4) |
GGCGAGATCACCAAGGTAGTC |
kan-5′-R (5) |
CAGCATCCATGTTGGAATTTAATCGC |
kan-3′-F (6) |
GATATGAATAAATTGCAGTTTCATTTG |
kan pBSV2 F (7) |
GCGATTAAATTCCAACATGGATGCTG |
kan pBSV2 R (8) |
ACTCATCGAGCATCAAATGAAACTGC |
flaB F |
CAGGTAACGGCACATATTCAGATGC |
flaB R |
CTTGGTTTGCTCCAACATGAACTC |
BB0419F_LIC |
GACGACGACAAGATGGAAATGATAATTAAAGATAAAGC |
BB0419R_LIC |
GAGGAGAAGCCCGGTTTAATATCTAAACTGATTTCTTCCAG |
rrp1 KI construction |
Rev_419+AatII+AscI |
GGCGCGCCTAGGACGTCTTAATATCTAAACTGATTTCTTCCAGAAAC |
Fwd_KanR+1kbDNST419 |
GAGTTTTTCTAAATTTATATTTAATAGACTTTAGTATTTATAAGTTATAGACATTCC |
Rev_1kbDNST419+AscI |
GGCGCGCCCTTTCCCAAGATCAACAGAACTTAG |
Fwd_KanR+AatII |
GACGTCCTTTAATAAAACAATATGTTGCGATGATTAAGG |
Rev_KanR+1kbDNST419 |
CTTATAAATACTAAAGTCTATTAAATATAAATTTAGAAAAACTCATCGAGCATCAAATG |
rrp1 OV construction |
pFlaB+BamHI F |
GTGGATCCTGAACTTAATACCTTGG |
pFlaB+ClaI+SalI R |
GTCGACATAATCGATTCCTCCATGATAAAATTTAAATTTC |
419+ClaI F |
ATCGATAATGATAATTAAAGATAAAGCTTTTG |
419+SalI R |
GTCGACCGCTTAATATCTAAACTGATTTCTTCC |
qPCR primers |
FlaB BB0147F-RT |
CAGGTAACGGCACATATTCAGATGC |
FlaB BB0147R-RT |
CTTGGTTTGCTCCAACATGAACTC |
BB0419F-RT |
TTGAGGTTGCAACAAATGGA |
BB0419R-RT |
CGGGATCGCTTTTTAGCTTT |
eno BB0337F-RT |
GCTTGAACTTGATGGCACCCCTAC |
eno BB0337R-RT |
GTACGCTCCAAGATATTGATAAGG |
tick RIB-3 (ITS2) |
CGGGATCCTTC(A,G)CTCGCCG(C,T)TACT |
tick RIB-4 (ITS2) |
CCATCGATGTGAA(C,T)TGCAGGACA |
BB0002F-RT |
GCGGAGCAGACAAAGGGATTGATT |
BB0002R-RT |
ACATGCTCCATGGCCGGAAA |
NagA BB0151F-RT |
GCAGCTGGTGGAGTATTTACAGGA |
NagA BB0151R-RT |
GTGTGTCCCGCTTGAAGGTTTATG |
NagB BB0152F-RT |
GGGCGGCTAATCATGTAGCACAAA |
NagB BB0152R-RT |
CAATCGGAGAGCTTCCTGTTGGA |
PtsG BB0645F-RT |
AGAACTTGCTGCCCAAGGTACAGA |
PtsG BB0645R-RT |
CCGGGCAAACCAAACATCATGGTA |