Table 1.
Oligonucleotides used in this study.
| Oligonucleotide | Sequence (5′→3′) |
|---|---|
| General primers | |
| KOverifyUp F (1) | CTTTAATGGGGCTTGGTATATGC |
| KOverifyDwn R (2) | GTTGATGCATCTGTTAAAATTG |
| aad1-5′-R (3) | TCCTTGAAGCTCGGGTATTA |
| pKFSS1-3′F (4) | GGCGAGATCACCAAGGTAGTC |
| kan-5′-R (5) | CAGCATCCATGTTGGAATTTAATCGC |
| kan-3′-F (6) | GATATGAATAAATTGCAGTTTCATTTG |
| kan pBSV2 F (7) | GCGATTAAATTCCAACATGGATGCTG |
| kan pBSV2 R (8) | ACTCATCGAGCATCAAATGAAACTGC |
| flaB F | CAGGTAACGGCACATATTCAGATGC |
| flaB R | CTTGGTTTGCTCCAACATGAACTC |
| BB0419F_LIC | GACGACGACAAGATGGAAATGATAATTAAAGATAAAGC |
| BB0419R_LIC | GAGGAGAAGCCCGGTTTAATATCTAAACTGATTTCTTCCAG |
| rrp1 KI construction | |
| Rev_419+AatII+AscI | GGCGCGCCTAGGACGTCTTAATATCTAAACTGATTTCTTCCAGAAAC |
| Fwd_KanR+1kbDNST419 | GAGTTTTTCTAAATTTATATTTAATAGACTTTAGTATTTATAAGTTATAGACATTCC |
| Rev_1kbDNST419+AscI | GGCGCGCCCTTTCCCAAGATCAACAGAACTTAG |
| Fwd_KanR+AatII | GACGTCCTTTAATAAAACAATATGTTGCGATGATTAAGG |
| Rev_KanR+1kbDNST419 | CTTATAAATACTAAAGTCTATTAAATATAAATTTAGAAAAACTCATCGAGCATCAAATG |
| rrp1 OV construction | |
| pFlaB+BamHI F | GTGGATCCTGAACTTAATACCTTGG |
| pFlaB+ClaI+SalI R | GTCGACATAATCGATTCCTCCATGATAAAATTTAAATTTC |
| 419+ClaI F | ATCGATAATGATAATTAAAGATAAAGCTTTTG |
| 419+SalI R | GTCGACCGCTTAATATCTAAACTGATTTCTTCC |
| qPCR primers | |
| FlaB BB0147F-RT | CAGGTAACGGCACATATTCAGATGC |
| FlaB BB0147R-RT | CTTGGTTTGCTCCAACATGAACTC |
| BB0419F-RT | TTGAGGTTGCAACAAATGGA |
| BB0419R-RT | CGGGATCGCTTTTTAGCTTT |
| eno BB0337F-RT | GCTTGAACTTGATGGCACCCCTAC |
| eno BB0337R-RT | GTACGCTCCAAGATATTGATAAGG |
| tick RIB-3 (ITS2) | CGGGATCCTTC(A,G)CTCGCCG(C,T)TACT |
| tick RIB-4 (ITS2) | CCATCGATGTGAA(C,T)TGCAGGACA |
| BB0002F-RT | GCGGAGCAGACAAAGGGATTGATT |
| BB0002R-RT | ACATGCTCCATGGCCGGAAA |
| NagA BB0151F-RT | GCAGCTGGTGGAGTATTTACAGGA |
| NagA BB0151R-RT | GTGTGTCCCGCTTGAAGGTTTATG |
| NagB BB0152F-RT | GGGCGGCTAATCATGTAGCACAAA |
| NagB BB0152R-RT | CAATCGGAGAGCTTCCTGTTGGA |
| PtsG BB0645F-RT | AGAACTTGCTGCCCAAGGTACAGA |
| PtsG BB0645R-RT | CCGGGCAAACCAAACATCATGGTA |