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Comparison of EGFP reporter expression in fat bodies of transgenic and non-transgenic female mosquitoes after 20E activation in an in vitro organ culture. Specific EGFP expression was observed only in the Vg-Gal4/UAS-EGFP hybrids (A), but not in the Vg-Gal4 driver (B), UAS-EGFP reporter (C), or non-transgenic UGAL females (D). Imaging was performed with a Zeiss AxioObserver A1 microscope using EGFP and Blue filters to obtain merged images of fat body preparations.
