Fig. 10.

LY29 and Wrt affect PIP levels in CV membranes. a Quantification of CV membrane PI and PI(4)P (n = 5–8); *P < 0.05, **P < 0.05 indicate significant difference from control and other conditions, respectively. b Detection and quantification of CV membrane PI, PI(3,4)P2 and PI(3,4,5)P3 following treatments with LY29 (blue line, n = 4–6) or Wrt (green line, n = 6–8, representative image). *P < 0.001 indicates significant difference from control for PI(3,4)P2 detected after LY29 treatments; *P < 0.05 indicates significant difference from control for PI(3,4,5)P3 detected after Wrt treatments