Fig. 6.

PE depletion or sequestration inhibits CV–CV fusion. a Ca²⁺–activity curves of CV from D609-treated eggs, including kinetics (inset) (n = 3–6). Vertical dotted lines indicate EC₅₀ of each curve. b Ca²⁺–activity curves of cinnamycin-treated CV, including kinetics (inset) (n = 3–5). Vertical dotted lines indicate EC₅₀ of each curve. c Detection and quantification of CV membrane PC, PE and lysoPE following D609 treatments (n = 4–5; representative image). *P < 0.05 indicates significant difference from control