Fig. 2.

Effects of PI3K inhibitors on giardial growth. Trophozoites were cultured in growth medium in the presence of LY 294002 or wortmannin as described in “Materials and Methods.” After overnight incubation at 37 °C, non-attached cells were decanted and the tubes were filled with cold PBS. Adherent trophozoites were detached by chilling the tubes in ice-cold water for ∼30 min and counted under phase-contrast microscopy. Data presented here are the mean ±SD of four separate experiments. Each experiment was carried out in duplicate. ● denotes LY 294002 and ○ indicates wortmannin.