TABLE-1.
Perdicted Giardia lamblia ORFs Containing PI3/PI4 Kinase Catalytic Motif.
| Designation (gORF) | GenBank Accession | Match to Pfam PI3/PI4 Kinase (motif location) | Other Pfam matchesd | Best BLASTp match species (Expectation Value) |
|---|---|---|---|---|
| gTOR (35280a) | AY095369.1 | 9e-64 (aa2883-3134) | Mammalian TOR H. sapien (8e-148) | |
| gPI3K-1 (14855) | XP771557.1 | 5.1e-47 (aa1776-2096) | PI3Kab | PI3 Kinase D. discoideum (2e-36) |
| gPI3K-2 | XP771095.1 | 6.7e-33 (aa1133-1590) | PI3Kac PI3KC2 | PI3 Kinase N. tabacum (5e-29) |
| gPI4K (16558) | XP768275.1 | 1.9e-24 | PI4 Kinase S. pombe (1e-45) | |
| PIK-like | XP779251.1 | 3.3e-6 (aa 2352-2590) | FATC | Ser/Thr prot kinase |
PI3Ka is an accessory domain believed to be involved in substrate presentation (Flanagan et al. 1993); PI3KC2 interacts with structural components of the PIK protein (Djordjevic and Driscoll, 2002); FATC domain is found at the C-terminal end of some PIK proteins and may act as an intracellular sensor (Bosotti et al. 2000.)
gORF 35280 uses an alternative start site for N-terminus from genbank reference.
HMMTOP (Tusnády and Simon, 2001) predicts two transmembrane helices, 1650-1667 and 1676-1693.
HMMTOP predicts three transmembrane helices, 1171-1189, 1564-1582, and 1605-1623.
gPI3K-1, giardial phosphatidylinositol 3 kinase; gPI3K-2; giardial phosphatidylinositol 3 kinase-2; gPI4K, giardial phosphatidylinositol 4 kinase; PIK, phosphatidylinositol kinase; D. discoideum, Dictostelium discoideum; N. tabacum, Nicotinica tabacum; H. sapiens; Homo sapiens; S. pombe, Saccahromyces pombe.