Figure 2.
Ab production in response to UVi Pn and Lm challenge. WT, p47phox-/-, and Nox2-/- mice were immunized by i.p. injection with 2 × 108 CFUs of UVi Pn or Lm on days 0 and 14 before serum collection. Serum was harvested on the indicated days. Serum titers for IgG and IgM specific for whole Pn (A), Lm (B), and PspA and LLO (C) were determined by ELISA. Absorbance values (Titer-1) represent the mean ± SEM. *P ≤ 0.05, **P ≤ 0.005, and ***P ≤ 0.0005. Results are representative of two individual experiments with five of each genotype per experiment.