Figure 3.

p47^phox in DCs is critical for IgM and IgG Abs in response to Lm. WT or p47^phox-/- BMDCs (1 × 10⁶), untreated or pulsed with 1 × 10⁸ CFUs of UVi Lm for 5 hours, were i.v. injected into WT recipient mice. The recipients also received a booster injection, 1 × 10⁸ CFUs of UVi Lm i.p., on day 14 after serum collection. A: Schematic timeline of adoptive transfer and serum collection. B: Serum titers for IgG and IgM specific for whole Lm were determined by ELISA. Absorbance values (Titer-1) represent the mean ± SEM. *P ≤ 0.05, **P ≤ 0.005, and ***P ≤ 0.0005. Results are representative of two individual experiments, with five and nine of each genotype in serial experiments.