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Aminoacylation of wild type and mutant tRNA[Ser]Sec isoforms with serine. Two µgs of wild type and mutant (UCCA, CCCA or ACCA) tRNA[Ser]Sec transcripts were aminoacylated with 3H-serine in the presence of SerRS as described in Materials and Methods. Results shown are counts per minute (CPM) of 3H-serine.
